FastQCFastQC Report
Mon 8 Aug 2022
30C_S114_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename30C_S114_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6925912
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA324480.4685014767730228No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT279480.40352808409924934No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA191620.2766711445366329No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG151150.21823840672535255No Hit
ATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATT128000.1848132058276224No Hit
TAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGA116760.16858429619088433No Hit
TACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTTATG115520.16679391825942924No Hit
AACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTG110780.15995005423112507No Hit
GATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGC100570.14520831336003115No Hit
TCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGG96740.13967835571690776No Hit
AAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCTTTT92490.13354197974216248No Hit
TTTAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA86110.12433019651419192No Hit
GATGGCCAGTGCCCCGAGGAACAGATGTGTTTCTGGATATGTTGATTCTA83080.11995532140749118No Hit
TGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATTCTTATT83010.1198542516855542No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA81560.11776066458828816No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG81410.11754408661270892No Hit
AAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTT74140.10704727406296817No Hit
TATGCAATAATAAATATGAAAAATGATCATAGCTTTTATGGAAACTGGAC73190.10567561355096627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)