FastQCFastQC Report
Mon 8 Aug 2022
29C_S113_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename29C_S113_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6409477
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA286370.44679152448787945No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA170140.2654506756167469No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT144660.225697042051949No Hit
CAGACTCAACCCACTTTTTGTACTGTACATAACATGCTTACAATCTGTTA104830.16355468628719627No Hit
TACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTTATG90290.14086952804417582No Hit
TATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA85830.13391108198063587No Hit
TAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGA84080.13118075000503163No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG81540.1272178681661546No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA80700.12590730881786455No Hit
AAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCTTTT77650.12114873023181144No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG77470.12086789608574927No Hit
ATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATT76210.11890205706331422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)