FastQCFastQC Report
Mon 8 Aug 2022
28C_S112_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename28C_S112_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7866616
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA1064151.352741763421527No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA635860.8083018161811889No Hit
TATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA341920.4346468672171109No Hit
TACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTTATG217060.2759255059608859No Hit
TTTAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA191940.24399309690469195No Hit
AAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCTTTT168610.21433612623267742No Hit
TAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATG166330.21143780248076174No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT155740.19797585136989018No Hit
TAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGA147350.18731052844069165No Hit
AAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTT131920.16769599533013943No Hit
TGAAAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCT129030.16402224285512346No Hit
ATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATT118030.1500391019467583No Hit
AGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGAT109630.13936106707127943No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG98260.124907584150542No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA94450.1200643326177355No Hit
AATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAAC88380.11234818122557401No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG83780.10650068593662128No Hit
GATGGCCAGTGCCCCGAGGAACAGATGTGTTTCTGGATATGTTGATTCTA81180.10319557990373497No Hit
AAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGA80820.10273794983764303No Hit
GTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGAA78780.10014471279645529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)