FastQCFastQC Report
Mon 8 Aug 2022
27C_S111_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename27C_S111_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6230766
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA170000.2728396476452494No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA103330.16583835759519774No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT100360.16107168845692488No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG76950.12350006403707024No Hit
AAGACAGTTTTAGGGAATCTATGTAATTCTACCTAGTTTGTTTTTATACT75900.12181487797808487No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA72120.11574820816573758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)