FastQCFastQC Report
Mon 8 Aug 2022
26C_S110_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename26C_S110_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6750352
Sequences flagged as poor quality0
Sequence length20-86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA362780.5374238261945451No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT248160.3676252734672207No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA212670.31505023738021365No Hit
ATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATT140090.20752991843980875No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG134070.19861186498126318No Hit
TACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTTATG116280.17225768374745495No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG112970.16735423574948388No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA108670.16098419756480847No Hit
TATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA107370.1590583720671159No Hit
TAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGA107050.15858432271383774No Hit
GTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGAA105040.15560670021355924No Hit
AACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTG103320.15305868493968908No Hit
GCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG102250.1514735824146652No Hit
TTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC100370.14868854246415594No Hit
TGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATTCTTATT99630.14759230333470016No Hit
AAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCTTTT94570.14009639793598913No Hit
CGGGGGCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA77730.11514955071972542No Hit
TTTAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA76020.11261634948814521No Hit
AAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTT75130.1112978997243403No Hit
CATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG73980.10959428486099687No Hit
TCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGG73650.10910542146542876No Hit
GATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGC73010.10815732275887242No Hit
ATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGA70960.10512044408943415No Hit
TGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATTCTT70290.10412790325600799No Hit
CATGTGTGCCGAGTACTGTTACAAAGATGGCCAGTGCCCCGAGGAACAGA67900.10058734714871165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)