FastQCFastQC Report
Mon 8 Aug 2022
25C_S109_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename25C_S109_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6373025
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA520920.8173826401120348No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA318530.4998097449798173No Hit
TATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA187870.29478936611734613No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT130360.20454964479191592No Hit
TACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTTATG100370.15749192887208194No Hit
TAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGA98210.15410264356408457No Hit
AAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCTTTT86990.1364971893253204No Hit
TAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATG77970.12234378493729431No Hit
TTTAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA72110.11314877942578289No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG71490.11217592901330214No Hit
ATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATT67110.10530321158319636No Hit
CAGACTCAACCCACTTTTTGTACTGTACATAACATGCTTACAATCTGTTA65920.10343596643666077No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG65580.10290246782336489No Hit
TGAAAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCT65470.102729865330828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)