FastQCFastQC Report
Mon 8 Aug 2022
24C_S108_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename24C_S108_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8057611
Sequences flagged as poor quality0
Sequence length20-86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA219040.27184236121599814No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT195830.24303729728327664No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG133200.1653095439827016No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA132430.16435392574796676No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA130700.16220688737641964No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG108740.1349531517468391No Hit
GCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG105390.1307955918944213No Hit
GTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGAA104640.12986479491253672No Hit
AAGACAGTTTTAGGGAATCTATGTAATTCTACCTAGTTTGTTTTTATACT91860.11400401434122347No Hit
TTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC91420.11345794677851785No Hit
CGGGGGCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA89680.11129849778054562No Hit
CATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG83960.10419961946537255No Hit
TATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA83590.10374042628764284No Hit
TAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGA81670.10135758601401829No Hit
AACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTG81170.10073705469276191No Hit
ATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGA80810.10029027214145732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)