FastQCFastQC Report
Mon 8 Aug 2022
23C_S107_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename23C_S107_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6456254
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA231850.3591091676380762No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA137230.21255359532013457No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT109010.16884403866390635No Hit
TATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA80360.12446846112312186No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG75730.1172971199708066No Hit
CAGACTCAACCCACTTTTTGTACTGTACATAACATGCTTACAATCTGTTA75560.11703380938854016No Hit
AAGACAGTTTTAGGGAATCTATGTAATTCTACCTAGTTTGTTTTTATACT70850.10973855737398189No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA67930.10521581090211134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)