FastQCFastQC Report
Mon 8 Aug 2022
22C_S106_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename22C_S106_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6992967
Sequences flagged as poor quality0
Sequence length20-86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT162970.2330484328039872No Hit
GCATGGAGATGAACTATGGAGCTTCTCGTGGCCAGAACGATGCCCCTTGC133740.19124929375471098No Hit
GCATGGAGATGAACTATGGAGCTTCTCGTAGTCAGAACGATGCCCCTTGC129990.1858867630863981No Hit
TAAGGACAACACGAACGGCCGGGAGCATTGCATGGAGATGAACTATGGAG108800.1555848897899847No Hit
GGGTCCAGGTTTAACTACCAGAACTGGGGCCCGGGTCAACCCTCTAACTA107860.15424068210246095No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG106600.1524388717979078No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA103940.1486350500438512No Hit
AGCGACGGGTCCAGGTTTAACTACCAGAACTGGGGCCCGGGTCAACCCTC100570.14381592248326067No Hit
ACTATGGAGCTTCTCGTAGTCAGAACGATGCCCCTTGCTGGTACAAACGT97500.1394257973761352No Hit
GGGTCCATGTTTAACTACCAGAACTGGGGCCCGGGTCAACCCTCTAACTC92060.13164655288663596No Hit
TCGTAGTCAGAACGATGCCCCTTGCTGGTACAAACGTCCCTTCCTGTGTT91420.1307313476525772No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG90840.1299019429092115No Hit
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA88460.12649852344505558No Hit
CATTTTGGAAGAGACGACGTTGGAGAAGCTCTCTAAAGATGAGATTGTTT87000.1244107115048591No Hit
ACTATGGAGCTTCTCGTGGCCAGAACGATGCCCCTTGCTGGTTTACATTT86930.12431061093238392No Hit
ATTGCATGGAGATGAACTATGGAGCTTCTCGTGGCCAGAACGATGCCCCT84990.12153639506664339No Hit
CGACGTTGGAGAAGCTCTCTAAAGATGAGATTGTTTTAAATAAAAGGCAC83560.1194914833717934No Hit
ATTGCATGGAGATGAACTATGGAGCTTCTCGTAGTCAGAACGATGCCCCT82040.11731787094090391No Hit
TATTAAGGACAACACGAACGGCCGGGAGCATTGCATGGAGATGAACTATG81160.11605946374407315No Hit
AAGACAGTTTTAGGGAATCTATGTAATTCTACCTAGTTTGTTTTTATACT81070.11593076300803365No Hit
GTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGAA80960.1157734621084298No Hit
GCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG79700.11397165180387667No Hit
TGTTATTAAGGACAACACGAACGGCCGGGAGCATTGCATGGAGATGAACT77730.11115453569278963No Hit
GCATTGCATGGAGATGAACTATGGAGCTTCTCGTGGCCAGAACGATGCCC77470.11078273356645327No Hit
AGCGACGGGTCCATGTTTAACTACCAGAACTGGGGCCCGGGTCAACCCTC75310.10769391590150504No Hit
GTTTAACTACCAGAACTGGGGCCCGGGTCAACCCTCTAACTATAACCATG73960.10576340486091239No Hit
TTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC73690.10537730265279388No Hit
GACGGGTCCAGGTTTAACTACCAGAACTGGGGCCCGGGTCAACCCTCTAA72050.10303208924051838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)