FastQCFastQC Report
Mon 8 Aug 2022
21C_S105_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename21C_S105_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8783504
Sequences flagged as poor quality0
Sequence length20-86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACAGTTTTAGGGAATCTATGTAATTCTACCTAGTTTGTTTTTATACT142690.1624522514021739No Hit
CAGACTCAACCCACTTTTTGTACTGTACATAACATGCTTACAATCTGTTA127720.14540893930258356No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG122360.13930659108255658No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA118190.13455905524719974No Hit
GCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG97500.11100353571877465No Hit
GTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGAA97030.11046844175172003No Hit
TTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC90200.10269250176239461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)