FastQCFastQC Report
Mon 8 Aug 2022
20C_S104_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename20C_S104_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7579366
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA441480.5824761596154612No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA279250.3684345102215673No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT196120.2587551518161282No Hit
TATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA155050.20456856153931607No Hit
CAGACTCAACCCACTTTTTGTACTGTACATAACATGCTTACAATCTGTTA141120.18618971560418113No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG104570.13796668481242363No Hit
TAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGA101050.13332249689485903No Hit
TACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTTATG86170.11369024797060863No Hit
AACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTG80090.10566846884026974No Hit
AAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCTTTT79010.10424354754738062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)