FastQCFastQC Report
Mon 8 Aug 2022
17C_S101_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename17C_S101_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711927
Sequences flagged as poor quality0
Sequence length20-86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCAC580611.5641740799320676No Hit
CTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTC472891.2739744073630759No Hit
ACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTAC336950.9077495327898419No Hit
GAGTCCAGAATCAGAACCATGCTACTCTGAGTCTGAAGAATTGTTCCAGA328370.8846348540798351No Hit
CAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACT272730.7347396648694869No Hit
TCAGAGTCCAGAATCAGAACCATGCTACTCTGAGTCTGAAGAATTGTTCC272330.733662057470419No Hit
TTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATA238950.6437357200182008No Hit
TCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGG230510.6209982038978676No Hit
TGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGC222920.6005506035005538No Hit
CTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGT204800.5517349883227768No Hit
AATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTG195880.5277043433235621No Hit
AAAGACATCAAAATGTAATTCCAATATATACAGGAGTTCAGAGTCCAGAA195840.5275965825836554No Hit
GACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAATTCA187700.5056672720126231No Hit
AAGACATCAAAATGTAATTCCAATATATACAGGAGTTCAGAGTCCAGAAT175220.47204592116170385No Hit
GAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAAT171940.46320954048934687No Hit
CCAAAGACATCAAAATGTAATTCCAATATATACAGGAGTTCAGAGTCCAG171140.461054325691211No Hit
TCCAAAGACATCAAAATGTAATTCCAATATATACAGGAGTTCAGAGTCCA170460.4592223931127956No Hit
AGTTCAGAGTCCAGAATCAGAACCATGCTACTCTGAGTCTGAAGAATTGT155800.4197280819369562No Hit
TACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTA154210.41544459252566124No Hit
TGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTG144680.3897705962428679No Hit
GGCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCC143440.3864300133057573No Hit
AGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTG138870.3741183487714064No Hit
GAAAGACCCCTTTACTGAGTCGACATCATCACATTTCACTAAATCATTCC136870.36873031177606674No Hit
AAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTAC134710.3629112318210999No Hit
ACCATGCTACTCTGAGTCTGAAGAATTGTTCCAGACCACAGAGGGATGAA130850.35251232042009445No Hit
CGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCT130810.3524045596801877No Hit
AGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTG126210.34001207459090654No Hit
TGAGGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAG120130.32363244212507414No Hit
AACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACT115690.31167099999542014No Hit
TGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTGGT114890.30951578519728434No Hit
GCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCG114470.308384297428263No Hit
AGACCCCTTTACTGAGTCGACATCATCACATTTCACTAAATCATTCCCTT113370.3054208770808262No Hit
CGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTC108790.2930822723614985No Hit
CCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCC108740.292947571436615No Hit
TGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCC103240.27813046969943106No Hit
CATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGCAATGTTGGG100650.2711529617904663No Hit
CAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTT96920.2611042727941579No Hit
GGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTG96890.2610234522392278No Hit
TGGTCCTCCAAAGACATCAAAATGTAATTCCAATATATACAGGAGTTCAG95840.25819473281667443No Hit
CTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGG92880.25022043806357186No Hit
ATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTCCACCAAGAT90650.24421277681376816No Hit
TTCAACTTTTCTAATTTAATTCTAATTATGGCAGCTGTGGTCCTCCAAAG88080.23728914927475675No Hit
CTGTGGTCCTCCAAAGACATCAAAATGTAATTCCAATATATACAGGAGTT84050.22643225472914744No Hit
ACATCAAAATGTAATTCCAATATATACAGGAGTTCAGAGTCCAGAATCAG82710.2228222699422699No Hit
CCTCCAAAGACATCAAAATGTAATTCCAATATATACAGGAGTTCAGAGTC82650.22266062883240967No Hit
TCCTCCAAAGACATCAAAATGTAATTCCAATATATACAGGAGTTCAGAGT80240.21616804425302547No Hit
CAACTTTTCTAATTTAATTCTAATTATGGCAGCTGTGGTCCTCCAAAGAC79830.21506349666898084No Hit
TCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTG77320.20830151023982962No Hit
GTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTCCAC76620.20641569729146075No Hit
AATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTG76460.20598465433183358No Hit
CTCTTCAACTTTTCTAATTTAATTCTAATTATGGCAGCTGTGGTCCTCCA75520.20345227694402393No Hit
TACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATAC73570.19819894087356782No Hit
TGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACC72870.19631312792519895No Hit
TGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAATTCACTTTC72210.1945350757167369No Hit
TCAAAATGTAATTCCAATATATACAGGAGTTCAGAGTCCAGAATCAGAAC71950.19383463090734274No Hit
ACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACCAGGCAGTGC71800.19343052813269226No Hit
AGAACCATGCTACTCTGAGTCTGAAGAATTGTTCCAGACCACAGAGGGAT70640.19030546667539527No Hit
GATGAAAGACCCCTTTACTGAGTCGACATCATCACATTTCACTAAATCAT69110.1861836183739605No Hit
TGTCATGATCTCTCTTCTTCCTGTGTCCCTCTGTCATGACATCTCTTCAA66190.17831708436076463No Hit
CTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGCAATGTT66040.17791298158611416No Hit
CTACAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACT65050.17524590327342104No Hit
CACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTGGTGTGTGACCA63390.17077383256728917No Hit
TTGGGGCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGA63210.1702889092377086No Hit
CCCCACTTGTGACTACAATGGACAATACACCCCTGAGCAATGTTGGGGCT63080.16993868683301153No Hit
TGGAACCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGC62700.168914959803897No Hit
CTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTCCACCAA62290.16781041221985238No Hit
TTCTTCCTGTGTCCCTCTGTCATGACATCTCTTCAACTTTTCTAATTTAA61640.166059300196367No Hit
GGTCCTCCAAAGACATCAAAATGTAATTCCAATATATACAGGAGTTCAGA60040.1617488706000953No Hit
CTTCAACTTTTCTAATTTAATTCTAATTATGGCAGCTGTGGTCCTCCAAA59730.16091372486581768No Hit
ATCTCTTCAACTTTTCTAATTTAATTCTAATTATGGCAGCTGTGGTCCTC59510.16032104079633033No Hit
AGATGAGGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGA59510.16032104079633033No Hit
TCCAGAATCAGAACCATGCTACTCTGAGTCTGAAGAATTGTTCCAGACCA59000.15894709136251872No Hit
CTAATTTAATTCTAATTATGGCAGCTGTGGTCCTCCAAAGACATCAAAAT57440.1547444225061538No Hit
GCCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACC56610.15250838715308787No Hit
ATCAGAACCATGCTACTCTGAGTCTGAAGAATTGTTCCAGACCACAGAGG56600.15248144696811117No Hit
GTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACCAGGCAG55650.14992212939532484No Hit
ACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAATTCAC55070.14835959866667636No Hit
AATATATACAGGAGTTCAGAGTCCAGAATCAGAACCATGCTACTCTGAGT54130.14582722127886674No Hit
GCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTAC54020.14553087924412306No Hit
CCCTCTGTCATGACATCTCTTCAACTTTTCTAATTTAATTCTAATTATGG53980.14542311850421627No Hit
AAAGACCCCTTTACTGAGTCGACATCATCACATTTCACTAAATCATTCCC53690.14464185313989203No Hit
CGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAATTCACT53320.14364506629575421No Hit
AATTGGAACCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTG52250.1407624665032475No Hit
CATCTCTTCAACTTTTCTAATTTAATTCTAATTATGGCAGCTGTGGTCCT52110.14038530391357373No Hit
ACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACT51240.13804150782060098No Hit
GGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGA50750.13672143875674278No Hit
AGATCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAA50320.13556301080274477No Hit
TGTAATTCCAATATATACAGGAGTTCAGAGTCCAGAATCAGAACCATGCT50020.13475480525344383No Hit
TACAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTG49430.13316533433981864No Hit
CTCTTCTTCCTGTGTCCCTCTGTCATGACATCTCTTCAACTTTTCTAATT49210.1325726502703313No Hit
AGGGATGAAAGACCCCTTTACTGAGTCGACATCATCACATTTCACTAAAT48750.13133340176140318No Hit
ACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTC48580.1308754186167993No Hit
CCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACCAGGCAGTGCT47550.1281005795641994No Hit
CAGCTGTGGTCCTCCAAAGACATCAAAATGTAATTCCAATATATACAGGA47530.12804669919424602No Hit
GAAAGACCCCTTTACTGAGTCTACATCATCACATTTCACTAAATCATTCC47190.1271307329050383No Hit
ATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTGGTGTGTGA46810.12610700587592374No Hit
CAGAGTCCAGAATCAGAACCATGCTACTCTGAGTCTGAAGAATTGTTCCA45480.12252396127402289No Hit
ATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCT45310.12206597812941904No Hit
TGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACCAGG45210.12179657627965206No Hit
GATCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAAC44480.11982994277635309No Hit
GACACATGGCCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGAC43770.11791718964300753No Hit
AAGATGAGGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTG43340.1167587616890095No Hit
AATGATTTCAGTGTCATGATCTCTCTTCTTCCTGTGTCCCTCTGTCATGA42780.11525011133031442No Hit
TGTGGTCCTCCAAAGACATCAAAATGTAATTCCAATATATACAGGAGTTC42330.114037803006363No Hit
AATGTAATTCCAATATATACAGGAGTTCAGAGTCCAGAATCAGAACCATG42170.11360676004673582No Hit
AGACCCCTTTACTGAGTCTACATCATCACATTTCACTAAATCATTCCCTT42160.11357981986175912No Hit
GACATCAAAATGTAATTCCAATATATACAGGAGTTCAGAGTCCAGAATCA41980.11309489653217858No Hit
TTCAGAGTCCAGAATCAGAACCATGCTACTCTGAGTCTGAAGAATTGTTC41700.11234057135283103No Hit
TTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGG40590.10935021082041753No Hit
GAAGATCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGC40510.10913468934060394No Hit
TAGCAACTGCTAGCAACTGTTCCACCAAGATGAGGACGTAGATGAAAAGC40100.10803014175655933No Hit
ACTTTTCTAATTTAATTCTAATTATGGCAGCTGTGGTCCTCCAAAGACAT39830.10730275676218849No Hit
GTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCA39330.10595574751335358No Hit
AGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAATTC39280.10582104658847008No Hit
TCCAATATATACAGGAGTTCAGAGTCCAGAATCAGAACCATGCTACTCTG39050.10520142233400602No Hit
CTTTTCTAATTTAATTCTAATTATGGCAGCTGTGGTCCTCCAAAGACATC39000.10506672140912253No Hit
GACATCTCTTCAACTTTTCTAATTTAATTCTAATTATGGCAGCTGTGGTC38650.10412381493493811No Hit
ATGTTGGGGCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGA38590.10396217382507791No Hit
TCTCTTCAACTTTTCTAATTTAATTCTAATTATGGCAGCTGTGGTCCTCC38540.10382747290019444No Hit
TTTCTAATTTAATTCTAATTATGGCAGCTGTGGTCCTCCAAAGACATCAA38360.10334254957061387No Hit
AACTGCTAGCAACTGTTCCACCAAGATGAGGACGTAGATGAAAAGCGTTG38350.10331560938563716No Hit
CAGTGTCATGATCTCTCTTCTTCCTGTGTCCCTCTGTCATGACATCTCTT38030.10245352346638284No Hit
CAGAGGGATGAAAGACCCCTTTACTGAGTCGACATCATCACATTTCACTA37800.10183389921191877No Hit
TGGACAATACACCCCTGAGCAATGTTCAGGCTCTACAGGTTACTGTTGGT37770.10175307865698867No Hit
GACTACAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTA37260.10037912922317707No Hit
TGGTTGTTGCCCGATGGCCAAAGCGGACCCCAGGCCAAGTACTCTGCCTG37260.10037912922317707No Hit
CGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAA37250.10035218903820037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)