FastQCFastQC Report
Mon 8 Aug 2022
16C_S100_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_S100_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7011611
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA299190.42670650154436696No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT255940.36502310239401475No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA182470.2602397651552546No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG135080.19265187415559706No Hit
ATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATT122810.17515232947178616No Hit
AACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTG106840.15237582347337866No Hit
TAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGA106280.15157714824738566No Hit
TATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA97160.1385701517097854No Hit
TACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTTATG94210.13436284471571514No Hit
GATGGCCAGTGCCCCGAGGAACAGATGTGTTTCTGGATATGTTGATTCTA89250.12728886414263427No Hit
TCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGG88600.1263618303981781No Hit
GATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGC84750.12087093821947625No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG83460.11903113278817093No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA82030.11699165855036738No Hit
AAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCTTTT80340.11458137081478137No Hit
TGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATTCTTATT71700.10225895304231795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)