FastQCFastQC Report
Mon 8 Aug 2022
15C_S99_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename15C_S99_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5774500
Sequences flagged as poor quality0
Sequence length20-86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACTCAACCCACTTTTTGTACTGTACATAACATGCTTACAATCTGTTA82590.1430253701619188No Hit
AAGACAGTTTTAGGGAATCTATGTAATTCTACCTAGTTTGTTTTTATACT81520.14117239587843103No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG75720.13112823621092734No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA72130.12491124772707593No Hit
GCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG59730.10343752705861979No Hit
GTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGAA59130.10239847605853321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)