FastQCFastQC Report
Mon 8 Aug 2022
12C_S96_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename12C_S96_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6786056
Sequences flagged as poor quality0
Sequence length20-86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCAC368820.5434968411695984No Hit
CTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTC331830.48898800717235463No Hit
ACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTAC196810.2900211846173978No Hit
AATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTG185070.27272100318653425No Hit
TGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGC184580.27199893428524613No Hit
CAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACT161550.23806169592470205No Hit
TTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATA155570.22924950810898115No Hit
TCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGG150360.22157199999528446No Hit
CTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGT137090.20201719526039869No Hit
GAGTCCAGAATCAGAACCATGCTACTCTGAGTCTGAAGAATTGTTCCAGA127330.1876347616347404No Hit
TCAGAGTCCAGAATCAGAACCATGCTACTCTGAGTCTGAAGAATTGTTCC121430.17894046261922977No Hit
TACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTA104230.15359437057401235No Hit
TCCAAAGACATCAAAATGTAATTCCAATATATACAGGAGTTCAGAGTCCA97690.14395696115681922No Hit
CATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGCAATGTTGGG96590.14233599015392742No Hit
CCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCC95490.1407150191510356No Hit
AAAGACATCAAAATGTAATTCCAATATATACAGGAGTTCAGAGTCCAGAA93870.13832777094677673No Hit
GGCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCC92460.13624998084306997No Hit
AGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTG78570.11578153790655427No Hit
CAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTT77970.11489737190497691No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA77480.11417530300368874No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG77010.11348270630245315No Hit
TGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTGGT76650.11295220670150673No Hit
AGTTCAGAGTCCAGAATCAGAACCATGCTACTCTGAGTCTGAAGAATTGT71920.10598203138907193No Hit
GCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCG71690.10564310108846729No Hit
CCAAAGACATCAAAATGTAATTCCAATATATACAGGAGTTCAGAGTCCAG71490.10534837908794152No Hit
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA71430.10525996248778378No Hit
GCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG68320.10067703537960783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)