FastQCFastQC Report
Mon 8 Aug 2022
11C_S95_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename11C_S95_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6991135
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT835191.1946414995562238No Hit
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA748141.0701266675582721No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA527470.7544840716135506No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG480840.6877853166903514No Hit
AACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTG348450.4984169237183948No Hit
ATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATT297540.4255961299560086No Hit
TATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA269720.3858028775012927No Hit
TGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATTCTTATT267040.38196945131226906No Hit
TAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGA241490.3454231680549725No Hit
TGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATTCTT237530.3397588517458181No Hit
GATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGC216610.3098352413449318No Hit
TACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTTATG210050.3004519294792619No Hit
TCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGG198350.28371644947494223No Hit
GATGGCCAGTGCCCCGAGGAACAGATGTGTTTCTGGATATGTTGATTCTA161890.23156468870934405No Hit
TTTAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA150970.21594490737197894No Hit
ACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGA141780.202799688462603No Hit
AAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTT136110.1946894173835865No Hit
AAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGA132750.1898833308182434No Hit
AAAGATGGCCAGTGCCCCGAGGAACAGATGTGTTTCTGGATATGTTGATT128250.18344660773965885No Hit
AATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAAC127350.18215926312394196No Hit
AAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCTTTT123460.17659507361823223No Hit
GTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTAT116090.16605315159841713No Hit
CATGTGTGCCGAGTACTGTTACAAAGATGGCCAGTGCCCCGAGGAACAGA111820.1599454165882936No Hit
TAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATG111000.15877250260508485No Hit
AGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGAT108830.15566857169830078No Hit
TGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAA108300.15491046875793416No Hit
TATGCAATAATAAATATGAAAAATGATCATAGCTTTTATGGAAACTGGAC105140.15039045877386145No Hit
TACAAAGATGGCCAGTGCCCCGAGGAACAGATGTGTTTCTGGATATGTTG98050.14024904396782498No Hit
TGAAAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCT97750.13981992909591934No Hit
TTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTG90820.12990737555489917No Hit
ATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCTTTTATGGAA89680.12827673904165776No Hit
CAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAA87450.12508698516049255No Hit
GTGCCCCGAGGAACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCA85950.12294141080096437No Hit
GGAACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCAT85220.12189723127932733No Hit
CTTTATTGGAAATTCTTATTAGTTATAAGAAATAAGTAGAAAATACAAAT82130.1174773480986993No Hit
GTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTT81430.1164760800642528No Hit
GAAGCTTTATTGGAAATTCTTATTAGTTATAAGAAATAAGTAGAAAATAC80530.11518873544853589No Hit
TTATTAGTTTTAAGAAATAAGTAGAAAATACAAATCATTACATGTACAAT79480.11368683339686618No Hit
TTATTAGTTATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAAT79400.113572402764358No Hit
CTTTATTGGAAATTCTTATTAGTTTTAAGAAATAAGTAGAAAATACAAAT77530.11089758672947955No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAACCATTGAAGCTT70650.101056552333777No Hit
TTCTTATTAGTTATAAGAAATAAGTAGAAAATACAAATCATTACATGTAC70160.10035566470966445No Hit
TTCTTATTAGTTTTAAGAAATAAGTAGAAAATACAAATCATTACATGTAC70020.10015541110277516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)