FastQCFastQC Report
Mon 8 Aug 2022
10C_S94_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename10C_S94_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8148503
Sequences flagged as poor quality0
Sequence length20-86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCAC352860.4330365958017074No Hit
CTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTC326310.4004539238679792No Hit
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA211940.26009685460016396No Hit
AATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTG199310.24459707507010797No Hit
TGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGC189110.23207943839500333No Hit
ACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTAC159820.19613418562894314No Hit
TTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATA146640.17995943549385696No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA146510.17979989698721346No Hit
TCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGG123350.15137749841903475No Hit
CAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACT123090.1510584214057478No Hit
CAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTT123050.15100933263447286No Hit
TGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTGGT115320.14152292758559457No Hit
CTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGT110440.13553409749005432No Hit
TATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA100780.12367915922716112No Hit
CCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCC100640.12350734852769889No Hit
TACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTA91470.11225374771292346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)