FastQCFastQC Report
Mon 8 Aug 2022
09C_S93_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename09C_S93_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6429025
Sequences flagged as poor quality0
Sequence length20-86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCAC561980.8741294364230968No Hit
CTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTC476570.741278809772866No Hit
ACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTAC400390.6227849479508946No Hit
CAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACT347520.5405485279649713No Hit
TGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGC323820.5036844622629403No Hit
AATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTG307350.47806626976874406No Hit
TCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGG274550.4270476471937813No Hit
CTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGT265680.41325084285719843No Hit
TTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATA238990.37173599418263265No Hit
CATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGCAATGTTGGG187160.29111723783933025No Hit
GGCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCC174610.2715963929211661No Hit
CCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCC172040.26759889718892055No Hit
TACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTA169430.2635391836242665No Hit
TGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTGGT145110.22571074151990386No Hit
GCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCG144830.22527521669304446No Hit
AGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTG135670.21102733307149993No Hit
CAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTT123790.19254863684617807No Hit
CTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGCAATGTT123180.19159981490194858No Hit
GACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAATTCA119920.18652906156065657No Hit
CACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTGGTGTGTGACCA115960.18036949615221592No Hit
TGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCC109280.1699791181399979No Hit
GAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAAT108660.16901474173766629No Hit
CCCCACTTGTGACTACAATGGACAATACACCCCTGAGCAATGTTGGGGCT104730.16290183970353203No Hit
CTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGG104550.16262185945769383No Hit
AATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTG101660.15812662106618033No Hit
CGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTC95420.1484206392104557No Hit
ATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTCCACCAAGAT95020.1477984608863708No Hit
AGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTG91060.14163889547793015No Hit
ATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTGGTGTGTGA90560.14086117257282402No Hit
ACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACCAGGCAGTGC89640.13943016242742873No Hit
TGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTG88960.13837245927648437No Hit
AAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTAC86580.13467049824817917No Hit
CTACAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACT83530.1299263885270317No Hit
TTGGGGCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGA82380.12813762584528757No Hit
GTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTCCAC80790.12566446700705006No Hit
GCCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACC79890.12426456577785899No Hit
TGAGGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAG77990.12130921873845567No Hit
TGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACC77350.12031373341991983No Hit
AACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACT76490.11897605002313726No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA76360.11877384206780965No Hit
TACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATAC75610.11760725771015045No Hit
CGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCT75090.11679842588884007No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG74840.11640956443628699No Hit
TCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTG71700.11152546459222044No Hit
GACACATGGCCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGAC70780.11009445444682515No Hit
GCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG70550.10973670191047633No Hit
CTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTCCACCAA70200.10919229587690203No Hit
TACAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTG67660.10524146351896284No Hit
GTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACCAGGCAG65500.1018817005689043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)