FastQCFastQC Report
Mon 8 Aug 2022
08C_S92_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename08C_S92_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6842200
Sequences flagged as poor quality0
Sequence length20-86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA126000.1841512963666657No Hit
CGTGTGCAGAGCCAGTGCTGCAGAAATGCAGTGACCTCGAAACAACTAAC109520.1600654760164859No Hit
AAGACAGTTTTAGGGAATCTATGTAATTCTACCTAGTTTGTTTTTATACT93720.1369734880593961No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA80430.11754991084738826No Hit
CAGACTCAACCCACTTTTTGTACTGTACATAACATGCTTACAATCTGTTA77120.11271228552220047No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT73760.10780158428575605No Hit
AGGTAGCCTAGTGGTTCGAGCCTAGCGTCAGTAACTGAAAGGTTGCTGGA70850.10354856625062114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)