FastQCFastQC Report
Mon 8 Aug 2022
07C_S91_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename07C_S91_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6108659
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA512920.8396605539775587No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT431570.7064889364425154No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA334420.5474523950346549No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG238830.3909696056041105No Hit
TAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGA177800.2910622445941081No Hit
AACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTG170170.278571778192235No Hit
TATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA163230.26721085593417476No Hit
GATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGC144030.23578006236720694No Hit
TCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGG125810.20595354888855313No Hit
ATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATT113630.18601463921950792No Hit
GATGGCCAGTGCCCCGAGGAACAGATGTGTTTCTGGATATGTTGATTCTA111680.18282244924786273No Hit
TACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTTATG108060.1768964350440907No Hit
AAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGA97850.16018245575665627No Hit
ACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGA96750.1583817332085487No Hit
TTTAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA91950.15052403481680676No Hit
AATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAAC91390.1496073033377702No Hit
TATGCAATAATAAATATGAAAAATGATCATAGCTTTTATGGAAACTGGAC88440.1447780928678455No Hit
AAAGATGGCCAGTGCCCCGAGGAACAGATGTGTTTCTGGATATGTTGATT84970.13909763173881534No Hit
AAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCTTTT79570.13025772104810565No Hit
GTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTAT74580.12208898876169057No Hit
AAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTT71910.11771814403128411No Hit
TAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATG65490.10720847243232925No Hit
TGAAAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCT61350.10043120756945181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)