FastQCFastQC Report
Mon 8 Aug 2022
05C_S89_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename05C_S89_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5142999
Sequences flagged as poor quality0
Sequence length20-86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCAC1058982.059070981736532No Hit
CTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTC860151.672467756653268No Hit
ACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTAC786411.5290883782011235No Hit
CAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACT646721.2574764257197018No Hit
TCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGG522911.0167413993275132No Hit
TGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGC483200.9395296401963136No Hit
CTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGT474430.9224773327780152No Hit
TTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATA431720.8394324012118222No Hit
GACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAATTCA430400.8368658053404249No Hit
AATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTG420590.8177913314779955No Hit
GAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAAT396000.7699787614191642No Hit
AGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTG338310.657806855494236No Hit
GGCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCC331420.6444100028018672No Hit
TGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTG325180.6322770041370803No Hit
CGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCT319510.6212523082349423No Hit
AAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTAC300570.5844255462620156No Hit
TGAGGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAG292780.5692787418391487No Hit
TACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTA278870.5422322656488948No Hit
TGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTGGT278320.5411628507024793No Hit
AACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACT270910.5267549147880449No Hit
GCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCG264080.5134747255443759No Hit
CGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTC262190.50979982691033No Hit
CATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGCAATGTTGGG235120.4571651676385704No Hit
CAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTT232630.45232363451752566No Hit
GGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTG231870.45084589750066056No Hit
AGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTG226660.44071562137188824No Hit
CCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCC226660.44071562137188824No Hit
ATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTCCACCAAGAT214450.41697460956146404No Hit
TGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCC204200.39704460374190237No Hit
AATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTG204040.396733501212036No Hit
GTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTCCAC192350.37400357262367734No Hit
TCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTG185160.3600234026878092No Hit
CTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGG184690.35910953900632686No Hit
TGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAATTCACTTTC182370.3545985523232651No Hit
ACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACCAGGCAGTGC170570.33165474074562334No Hit
TTGGGGCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGA156550.3043943815660863No Hit
CTACAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACT155810.30295553236545447No Hit
CTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTCCACCAA148730.2891892454188694No Hit
TGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACC147290.28638932265007244No Hit
CACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTGGTGTGTGACCA146990.2858060054065731No Hit
CCCCACTTGTGACTACAATGGACAATACACCCCTGAGCAATGTTGGGGCT146060.283997721951725No Hit
TACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATAC142890.2778340030787484No Hit
CTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGCAATGTT140830.27382855800671946No Hit
AGATGAGGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGA139350.2709508596054559No Hit
ACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAATTCAC134190.2609178030172668No Hit
CGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAATTCACT133250.2590900756543021No Hit
GCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTAC133170.2589345243893689No Hit
TACAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTG128460.24977644366642884No Hit
AGATCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAA125560.24413771031260167No Hit
ACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTC122200.23760455718540874No Hit
GCCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACC121130.2355240590169277No Hit
GTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACCAGGCAG121070.23540739556822782No Hit
CCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACCAGGCAGTGCT117790.22902979370596804No Hit
GATCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAAC110920.21567182882983255No Hit
ACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACT107640.2092942269675728No Hit
ATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTGGTGTGTGA105550.2052304501711939No Hit
GACACATGGCCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGAC103980.20217775659688053No Hit
ATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCT102870.2000194827959329No Hit
TTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGG102440.19918339474691715No Hit
GGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGA101550.1974528869245357No Hit
AAGATGAGGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTG101120.19661679887551992No Hit
GAAGATCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGC100570.1955473839291044No Hit
AGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAATTC100560.19552794002098778No Hit
CTGGACAGAAGATCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAAC96410.18745871815257986No Hit
ATGTTGGGGCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGA92880.18059501858740395No Hit
GACTACAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTA90920.17678401259654145No Hit
GTTGGTGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCA89900.1748007339686436No Hit
AACTGCTAGCAACTGTTCCACCAAGATGAGGACGTAGATGAAAAGCGTTG89510.17404242155209443No Hit
CGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAA89260.17355632384917827No Hit
TGTGTGACCAGTACTGGACAGAAGATCCCGGGTACTGATACTCCACCAGG87080.16931755187974953No Hit
GCTAGCAACTGCTAGCAACTGTTCCACCAAGATGAGGACGTAGATGAAAA85130.16552598979700364No Hit
AAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTAATT85060.16538988244018715No Hit
ACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTACAGGAACTA79990.15553182102504784No Hit
AGCAACTGCTAGCAACTGTTCCACCAAGATGAGGACGTAGATGAAAAGCG78210.15207080538028492No Hit
TAGCAACTGCTAGCAACTGTTCCACCAAGATGAGGACGTAGATGAAAAGC77700.151079166066336No Hit
ATCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTAGCAACT76940.14960142904947094No Hit
ACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTACTGTTGGTGTG75740.14726816007547347No Hit
ACCAAGATGAGGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGAC74850.145537652253092No Hit
ATGGCCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATAC74590.14503211064205923No Hit
GTACTGGACAGAAGATCCCGGGTACTGATACTCCACCAGGCAGTGCTAGC73770.1434377101764943No Hit
CCAAGATGAGGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACT70500.1370795522223512No Hit
TGCTAGCAACTGCTAGCAACTGTTCCACCAAGATGAGGACGTAGATGAAA68210.13262689726363938No Hit
ACCAGGCAGTGCTAGCAACTGCTAGCAACTGTTCCACCAAGATGAGGACG68160.13252967772305616No Hit
AGTGCTAGCAACTGCTAGCAACTGTTCCACCAAGATGAGGACGTAGATGA67210.1306825064519748No Hit
AGGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTT66750.1297880866786091No Hit
TAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTT66450.12920476943510975No Hit
CACATGGCCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAA63310.12309938228648304No Hit
CAGAAGATCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCTA61900.12035779124203601No Hit
CGCGACACATGGCCCAATTGGAGGCTTCATCCCCACTTGTGACTACAATG61880.12031890342580273No Hit
AGCGTTGGAACGAAGACGTTGAAGACTTGACAGTTGTCTTTGCTGTACTA61160.11891894204140425No Hit
GGGCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGACAGAAGATCC60510.1176550880138223No Hit
CCAATTGGAGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCCC58050.11287188661712748No Hit
CAGTGCTAGCAACTGCTAGCAACTGTTCCACCAAGATGAGGACGTAGATG56440.10974141741034754No Hit
GAGTCCAGAATCAGAACCATGCTACTCTGAGTCTGAAGAATTGTTCCAGA55450.10781647050679963No Hit
ACTGGACAGAAGATCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAA55060.10705815809025046No Hit
AGGCTTCATCCCCACTTGTGACTACAATGGACAATACACCCCTGAGCAAT54070.10513321118670255No Hit
GCAATGTTGGGGCTCTACAGGTTACTGTTGGTGTGTGACCAGTACTGGAC53600.1042193475052202No Hit
ATGAGGACGTAGATGAAAAGCGTTGGAACGAAGACGTTGAAGACTTGACA53160.10336381554808781No Hit
GGCAGTGCTAGCAACTGCTAGCAACTGTTCCACCAAGATGAGGACGTAGA52890.10283883002893837No Hit
ACAGAAGATCCCGGGTACTGATACTCCACCAGGCAGTGCTAGCAACTGCT52770.10260550313153861No Hit
ACTACAATGGACAATACACCCCTGAGCAATGTTGGGGCTCTACAGGTTAC52130.10136109301207331No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)