FastQCFastQC Report
Mon 8 Aug 2022
04C_S88_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename04C_S88_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6956999
Sequences flagged as poor quality0
Sequence length20-86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG113660.16337504145106244No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA112400.1615639157056081No Hit
AAGACAGTTTTAGGGAATCTATGTAATTCTACCTAGTTTGTTTTTATACT99000.1423027371428399No Hit
GCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG98810.14202963087963646No Hit
GTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGAA90820.1305447938112396No Hit
CGGGGGCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA89240.1282736996224953No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT87950.1264194518354825No Hit
TTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC82930.11920369688137084No Hit
CAGACTCAACCCACTTTTTGTACTGTACATAACATGCTTACAATCTGTTA81850.11765130338526712No Hit
CATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG72580.10432659254371031No Hit
ATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGA71420.10265920693678408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)