FastQCFastQC Report
Mon 8 Aug 2022
03C_S87_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename03C_S87_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5841120
Sequences flagged as poor quality0
Sequence length20-86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT156160.26734598844057306No Hit
CGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGA113300.19396964965623031No Hit
TCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACG109730.18785780809159885No Hit
GCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG94090.16108212123702303No Hit
GTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACGAA91990.1574869203166516No Hit
TTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC88650.15176883885282275No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG87640.15003971841016792No Hit
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA85880.147026597638809No Hit
AAGACAGTTTTAGGGAATCTATGTAATTCTACCTAGTTTGTTTTTATACT73800.12634563234448187No Hit
CGGGGGCATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA73290.1254725121209631No Hit
ATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGA72250.1236920316651601No Hit
CATTCGTATTGTGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG71690.12273331141972772No Hit
CAGACTCAACCCACTTTTTGTACTGTACATAACATGCTTACAATCTGTTA70990.12153491111293725No Hit
AACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTG65920.11285506889089764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)