FastQCFastQC Report
Mon 8 Aug 2022
02C_S86_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename02C_S86_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6320721
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACAGTTTTAGGGAATCTATGTAATTCTACCTAGTTTGTTTTTATACT109710.17357197066600472No Hit
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA109320.1729549524492538No Hit
CAGACTCAACCCACTTTTTGTACTGTACATAACATGCTTACAATCTGTTA72970.11544569045208608No Hit
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT63990.10123845048689857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)