FastQCFastQC Report
Mon 8 Aug 2022
01C_S85_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename01C_S85_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5193211
Sequences flagged as poor quality0
Sequence length20-86
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTT394280.7592219919429424No Hit
AAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAA267090.514306081536067No Hit
AGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAG215680.41531145181661216No Hit
AAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGA171180.3296226554245533No Hit
AACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTG148950.2868167690471271No Hit
TAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGA136060.2619959019573824No Hit
GATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGC128070.24661043042541503No Hit
ATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATT113210.21799614920325786No Hit
TCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGG104910.2020137444829413No Hit
TACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTTATG95640.18416351656037083No Hit
GATGGCCAGTGCCCCGAGGAACAGATGTGTTTCTGGATATGTTGATTCTA87480.16845069457027648No Hit
AGGTAGCCTAGTGGTTCGAGCCTAGCGTCAGTAACTGAAAGGTTGCTGGA83820.1614030317658959No Hit
ACAGATGTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGA83690.16115270494497527No Hit
ATCAGGACATCCTAATGGTGCAGCCGCTATTAAGGGTTCGTTTGTTCAAC79010.15214093939183293No Hit
TATAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA76820.14792389525478553No Hit
AAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGA74090.14266703201545247No Hit
TGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATTCTTATT74040.14257075246894454No Hit
AATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTATGAAAAC67910.13076688006707218No Hit
TATGCAATAATAAATATGAAAAATGATCATAGCTTTTATGGAAACTGGAC67540.1300544114229135No Hit
AAAGATGGCCAGTGCCCCGAGGAACAGATGTGTTTCTGGATATGTTGATT67180.1293611986880564No Hit
GTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTAT62680.12069603950234259No Hit
TGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTTATTGGAAATTCTT60280.11607462126996188No Hit
AAATACAAATCATTACATGTACAATTATGTCTATGAAAACTGATGATTTT60240.11599759763275554No Hit
AAACTGATGATTTTATGCAATAATAAATATGAAAAATGATCATAGCTTTT59240.1140720067025969No Hit
GAGATTTTAACTCCCACCCTTAACTCCCAAAGCTAAGATTCTAAATTAAA58300.1122619512282478No Hit
GTGTTTCTGGATATGTTGATTCTATGCCTGCAGTGAGCCATTGAAGCTTT54410.10477140250993075No Hit
TTTAAGAAATAAGTAGAAAATACAAATCATTACATGTACAATTATGTCTA52300.10070840564729605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)