FastQCFastQC Report
Fri 21 Oct 2022
09A_S40_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename09A_S40_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533407
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGAAGAGCTACGAGCTGCCCGACGGACAGGTCATCACAATCGGCAACG416277.803984574630629No Hit
CCGTCTCTTACGTGTGGCAGATCCACAGAACAGAAATGGCAAAGTCAAAG101271.8985502627449584No Hit
GATGCCCGACCAGAAGTGAACGTGTTCACTCTAGAACCTTATGACCTCAT40400.7573953847624797No Hit
CTTGCCTGAAGGAACCCCCGTCCCCCTAATCCCTGTACTAATTATTATCG28580.5358009924879126No Hit
GGCTGGGGGGACAGGGAGGCGTCTGTCTTCATCGAAGTGTCAAGACGGGG25800.4836831912592073No Hit
GTCAACCCTGAGCTCACCGTTAGACCCAACAAGCCAGTGATGGCATTTGG24390.4572493424345762No Hit
ACCCATCCATCCATACCACACAAACACACACACCTTGATGTTGCTTGCCT21300.3993198439465549No Hit
CACTGGGGATTCCCTCATCATCATCCTTCTCTCTGTTCACATACACAACC13290.24915308573003353No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13230.24802824109919816No Hit
CATTTACCAATGTCATTCCTGTATAAGAAACCTCAAATACTACTCCTGTC12450.2334052608983384No Hit
GCCTACACACACACACACACACACACACACACACACACCCACACACACAC11480.21522027269983332No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAGATCGGAAGAGCACAC10730.20115971481439124No Hit
CATGCATGGGCCTTCAAATAAAACAATTCAATTGAAAAAAAAAAAAAAAA10340.1938482247139614No Hit
TTAATGATGGCCTTTTTAAAATATTTTTATACACTGCTGATTTACCAATA10080.18897389798034145No Hit
GGGCTTCTCTGAGGTGGAGCTGGTGCAGATGGTTGTCGACGGCGTCAAGA9720.18222483019532928No Hit
AATTTGTTTGTCACATAGTGTGTTGGAAAGCAGATTTGTACTTTCTTTAC9620.180350089143937No Hit
TCCTGGGTTCTTTCTTGATAATCTTCTCCTTAAGAGCTTTTGGTTTAATT8560.16047783399917886No Hit
GCCTCTGACATACTGTACAACTGTGGCCTGTTCCAATGAGAAAGAGAGAG8080.15147907695249593No Hit
CTCTGGGAAGAAGAAAGACATGGTGTATGTTTCATGATTACCTACTCATG7120.13348156285913007No Hit
TACAGTTTAATTGAATCTCTCTTCTCTTTCTCTCTCTTTCTTCATCACCC7090.1329191405437124No Hit
TGACGCACCGACGAACCCAAGCACCAGACACCTGAAGACACCTGAGACGA6980.13085692538718088No Hit
GTGCCTCAACTTTTTTGATGATTTTTGATGTTATGCCAGACACACACACA6830.12804481381009247No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAGATCGGAAGAGCACACG6810.12766986559981403No Hit
TACAGTTTAATTGAATCTCTCTTCTCTTTCTCTCTCTTTTTTCATCACCC6660.1248577540227256No Hit
CGCAGGGATACCACGACGGTACTCTGACTACCCCGACGCCTATACACTGT6140.11510910055548577No Hit
GCCTACACACACACACACACACACACACACACACACCCACACACACACAA5820.10910992919103049No Hit
GATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5640.1057353952985244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)