[2023-04-04 15:14:27,492] multiqc [DEBUG ] This is MultiQC v1.12 [2023-04-04 15:14:27,492] multiqc [DEBUG ] No MultiQC config found: /opt/anaconda/anaconda3/lib/python3.9/site-packages/multiqc_config.yaml [2023-04-04 15:14:27,492] multiqc [DEBUG ] No MultiQC config found: /home/shared/8TB_HDD_02/mattgeorgephd/.multiqc_config.yaml [2023-04-04 15:14:27,493] multiqc [DEBUG ] No MultiQC config found: multiqc_config.yaml [2023-04-04 15:14:27,493] multiqc [DEBUG ] Command used: /opt/anaconda/anaconda3/bin/multiqc . [2023-04-04 15:14:28,138] multiqc [WARNING] MultiQC Version v1.14 now available! [2023-04-04 15:14:28,139] multiqc [DEBUG ] Command : /opt/anaconda/anaconda3/bin/multiqc . [2023-04-04 15:14:28,139] multiqc [DEBUG ] Working dir : /home/shared/8TB_HDD_02/mattgeorgephd/USDA-NRSP-8-gigas-rDNA/fastqc/untrimmed [2023-04-04 15:14:28,139] multiqc [DEBUG ] Template : default [2023-04-04 15:14:28,139] multiqc [DEBUG ] Running Python 3.9.7 (default, Sep 16 2021, 13:09:58) [GCC 7.5.0] [2023-04-04 15:14:28,140] multiqc [DEBUG ] Analysing modules: custom_content, ccs, ngsderive, purple, conpair, lima, peddy, somalier, methylQA, mosdepth, phantompeakqualtools, qualimap, preseq, quast, qorts, rna_seqc, rockhopper, rsem, rseqc, busco, bustools, goleft_indexcov, gffcompare, disambiguate, supernova, deeptools, sargasso, verifybamid, mirtrace, happy, mirtop, sambamba, homer, hops, macs2, theta2, snpeff, gatk, htseq, bcftools, featureCounts, fgbio, dragen, dedup, pbmarkdup, damageprofiler, biobambam2, jcvi, mtnucratio, picard, vep, sentieon, prokka, qc3C, nanostat, samblaster, samtools, sexdeterrmine, eigenstratdatabasetools, bamtools, jellyfish, vcftools, longranger, stacks, varscan2, snippy, bbmap, bismark, biscuit, hicexplorer, hicup, hicpro, salmon, kallisto, slamdunk, star, hisat2, tophat, bowtie2, bowtie1, snpsplit, odgi, pangolin, kat, leehom, adapterRemoval, clipandmerge, cutadapt, flexbar, kaiju, kraken, malt, trimmomatic, sickle, skewer, sortmerna, biobloomtools, fastq_screen, afterqc, fastp, fastqc, pychopper, pycoqc, minionqc, multivcfanalyzer, clusterflow, checkqc, bcl2fastq, bclconvert, interop, ivar, flash, seqyclean, optitype, whatshap [2023-04-04 15:14:28,140] multiqc [DEBUG ] Using temporary directory for creating report: /tmp/tmpecqnbhwg [2023-04-04 15:14:28,609] multiqc [INFO ] Search path : /home/shared/8TB_HDD_02/mattgeorgephd/USDA-NRSP-8-gigas-rDNA/fastqc/untrimmed [2023-04-04 15:14:28,673] multiqc [DEBUG ] File ignored by ccs/v4 because it exceeded search pattern filesize limit: F053n03_R1_001_fastqc.html [2023-04-04 15:14:28,674] multiqc [DEBUG ] File ignored by hicexplorer because it exceeded search pattern filesize limit: 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] File ignored by rseqc/infer_experiment because it exceeded search pattern filesize limit: F142n03_R1_001_fastqc.html [2023-04-04 15:14:29,836] multiqc [DEBUG ] File ignored by nanostat because it exceeded search pattern filesize limit: F142n03_R1_001_fastqc.html [2023-04-04 15:14:29,847] multiqc [DEBUG ] File ignored by ccs/v4 because it exceeded search pattern filesize limit: F052n06_R2_001_fastqc.html [2023-04-04 15:14:29,847] multiqc [DEBUG ] File ignored by hicexplorer because it exceeded search pattern filesize limit: F052n06_R2_001_fastqc.html [2023-04-04 15:14:29,847] multiqc [DEBUG ] File ignored by lima/summary because it exceeded search pattern filesize limit: F052n06_R2_001_fastqc.html [2023-04-04 15:14:29,848] multiqc [DEBUG ] File ignored by vep/vep_txt because it exceeded search pattern filesize limit: F052n06_R2_001_fastqc.html [2023-04-04 15:14:29,848] multiqc [DEBUG ] File ignored by longranger/invocation because it exceeded search pattern filesize limit: 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] File ignored by rseqc/infer_experiment because it exceeded search pattern filesize limit: F052n08_R1_001_fastqc.html [2023-04-04 15:14:30,512] multiqc [DEBUG ] File ignored by nanostat because it exceeded search pattern filesize limit: F052n08_R1_001_fastqc.html [2023-04-04 15:14:30,518] multiqc [DEBUG ] File ignored by ccs/v4 because it exceeded search pattern filesize limit: F143n06_R2_001_fastqc.html [2023-04-04 15:14:30,519] multiqc [DEBUG ] File ignored by hicexplorer because it exceeded search pattern filesize limit: F143n06_R2_001_fastqc.html [2023-04-04 15:14:30,519] multiqc [DEBUG ] File ignored by lima/summary because it exceeded search pattern filesize limit: F143n06_R2_001_fastqc.html [2023-04-04 15:14:30,520] multiqc [DEBUG ] File ignored by vep/vep_txt because it exceeded search pattern filesize limit: F143n06_R2_001_fastqc.html [2023-04-04 15:14:30,520] multiqc [DEBUG ] File ignored by longranger/invocation because it exceeded search pattern filesize limit: 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] File ignored by rseqc/infer_experiment because it exceeded search pattern filesize limit: F052n02_R1_001_fastqc.html [2023-04-04 15:14:31,217] multiqc [DEBUG ] File ignored by nanostat because it exceeded search pattern filesize limit: F052n02_R1_001_fastqc.html [2023-04-04 15:14:31,223] multiqc [DEBUG ] File ignored by ccs/v4 because it exceeded search pattern filesize limit: F052n05_R1_001_fastqc.html [2023-04-04 15:14:31,224] multiqc [DEBUG ] File ignored by hicexplorer because it exceeded search pattern filesize limit: F052n05_R1_001_fastqc.html [2023-04-04 15:14:31,224] multiqc [DEBUG ] File ignored by lima/summary because it exceeded search pattern filesize limit: F052n05_R1_001_fastqc.html [2023-04-04 15:14:31,225] multiqc [DEBUG ] File ignored by vep/vep_txt because it exceeded search pattern filesize limit: F052n05_R1_001_fastqc.html [2023-04-04 15:14:31,225] multiqc [DEBUG ] File ignored by longranger/invocation because it exceeded search pattern filesize limit: 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limit: F142n07_R1_001_fastqc.html [2023-04-04 15:14:31,731] multiqc [DEBUG ] File ignored by nanostat because it exceeded search pattern filesize limit: F142n07_R1_001_fastqc.html [2023-04-04 15:14:31,737] multiqc [DEBUG ] File ignored by ccs/v4 because it exceeded search pattern filesize limit: F142n05_R1_001_fastqc.html [2023-04-04 15:14:31,738] multiqc [DEBUG ] File ignored by hicexplorer because it exceeded search pattern filesize limit: F142n05_R1_001_fastqc.html [2023-04-04 15:14:31,738] multiqc [DEBUG ] File ignored by lima/summary because it exceeded search pattern filesize limit: F142n05_R1_001_fastqc.html [2023-04-04 15:14:31,739] multiqc [DEBUG ] File ignored by vep/vep_txt because it exceeded search pattern filesize limit: F142n05_R1_001_fastqc.html [2023-04-04 15:14:31,739] multiqc [DEBUG ] File ignored by longranger/invocation because it exceeded search pattern filesize limit: F142n05_R1_001_fastqc.html [2023-04-04 15:14:31,739] multiqc [DEBUG ] File ignored by rockhopper because it exceeded search pattern filesize limit: F142n05_R1_001_fastqc.html [2023-04-04 15:14:31,739] multiqc [DEBUG ] File ignored by rseqc/bam_stat because it exceeded search pattern filesize limit: F142n05_R1_001_fastqc.html [2023-04-04 15:14:31,739] multiqc [DEBUG ] File ignored by rseqc/junction_annotation because it exceeded search pattern filesize limit: F142n05_R1_001_fastqc.html [2023-04-04 15:14:31,739] multiqc [DEBUG ] File ignored by rseqc/read_distribution because it exceeded search pattern filesize limit: F142n05_R1_001_fastqc.html [2023-04-04 15:14:31,739] multiqc [DEBUG ] File ignored by rseqc/infer_experiment because it exceeded search pattern filesize limit: F142n05_R1_001_fastqc.html [2023-04-04 15:14:31,779] multiqc [DEBUG ] File ignored by nanostat because it exceeded search pattern filesize limit: F142n05_R1_001_fastqc.html [2023-04-04 15:14:31,784] multiqc [DEBUG ] File ignored by ccs/v4 because it exceeded search pattern filesize limit: 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F052n02_R2_001_fastqc.html [2023-04-04 15:14:31,840] multiqc [DEBUG ] File ignored by vep/vep_txt because it exceeded search pattern filesize limit: F052n02_R2_001_fastqc.html [2023-04-04 15:14:31,840] multiqc [DEBUG ] File ignored by longranger/invocation because it exceeded search pattern filesize limit: F052n02_R2_001_fastqc.html [2023-04-04 15:14:31,840] multiqc [DEBUG ] File ignored by rockhopper because it exceeded search pattern filesize limit: F052n02_R2_001_fastqc.html [2023-04-04 15:14:31,840] multiqc [DEBUG ] File ignored by rseqc/bam_stat because it exceeded search pattern filesize limit: F052n02_R2_001_fastqc.html [2023-04-04 15:14:31,840] multiqc [DEBUG ] File ignored by rseqc/junction_annotation because it exceeded search pattern filesize limit: F052n02_R2_001_fastqc.html [2023-04-04 15:14:31,840] multiqc [DEBUG ] File ignored by rseqc/read_distribution because it exceeded search pattern filesize limit: F052n02_R2_001_fastqc.html [2023-04-04 15:14:31,840] multiqc [DEBUG ] File ignored by rseqc/infer_experiment because it exceeded search pattern filesize limit: F052n02_R2_001_fastqc.html [2023-04-04 15:14:31,882] multiqc [DEBUG ] File ignored by nanostat because it exceeded search pattern filesize limit: F052n02_R2_001_fastqc.html [2023-04-04 15:14:31,887] multiqc [DEBUG ] File ignored by ccs/v4 because it exceeded search pattern filesize limit: F143n07_R1_001_fastqc.html [2023-04-04 15:14:31,887] multiqc [DEBUG ] File ignored by hicexplorer because it exceeded search pattern filesize limit: F143n07_R1_001_fastqc.html [2023-04-04 15:14:31,888] multiqc [DEBUG ] File ignored by lima/summary because it exceeded search pattern filesize limit: F143n07_R1_001_fastqc.html [2023-04-04 15:14:31,888] multiqc [DEBUG ] File ignored by vep/vep_txt because it exceeded search pattern filesize limit: F143n07_R1_001_fastqc.html [2023-04-04 15:14:31,888] multiqc [DEBUG ] File ignored by longranger/invocation because it exceeded search pattern filesize limit: 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File ignored by ccs/v4 because it exceeded search pattern filesize limit: F143n01_R1_001_fastqc.html [2023-04-04 15:14:31,934] multiqc [DEBUG ] File ignored by hicexplorer because it exceeded search pattern filesize limit: F143n01_R1_001_fastqc.html [2023-04-04 15:14:31,934] multiqc [DEBUG ] File ignored by lima/summary because it exceeded search pattern filesize limit: F143n01_R1_001_fastqc.html [2023-04-04 15:14:31,935] multiqc [DEBUG ] File ignored by vep/vep_txt because it exceeded search pattern filesize limit: F143n01_R1_001_fastqc.html [2023-04-04 15:14:31,935] multiqc [DEBUG ] File ignored by longranger/invocation because it exceeded search pattern filesize limit: F143n01_R1_001_fastqc.html [2023-04-04 15:14:31,935] multiqc [DEBUG ] File ignored by rockhopper because it exceeded search pattern filesize limit: F143n01_R1_001_fastqc.html [2023-04-04 15:14:31,935] multiqc [DEBUG ] File ignored by rseqc/bam_stat because it exceeded search pattern filesize limit: 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limit: F143n05_R1_001_fastqc.html [2023-04-04 15:14:31,983] multiqc [DEBUG ] File ignored by rseqc/infer_experiment because it exceeded search pattern filesize limit: F143n05_R1_001_fastqc.html [2023-04-04 15:14:32,022] multiqc [DEBUG ] File ignored by nanostat because it exceeded search pattern filesize limit: F143n05_R1_001_fastqc.html [2023-04-04 15:14:32,029] multiqc [DEBUG ] File ignored by ccs/v4 because it exceeded search pattern filesize limit: F053n01_R1_001_fastqc.html [2023-04-04 15:14:32,029] multiqc [DEBUG ] File ignored by hicexplorer because it exceeded search pattern filesize limit: F053n01_R1_001_fastqc.html [2023-04-04 15:14:32,029] multiqc [DEBUG ] File ignored by lima/summary because it exceeded search pattern filesize limit: F053n01_R1_001_fastqc.html [2023-04-04 15:14:32,030] multiqc [DEBUG ] File ignored by vep/vep_txt because it exceeded search pattern filesize limit: F053n01_R1_001_fastqc.html [2023-04-04 15:14:32,030] multiqc [DEBUG ] File ignored by longranger/invocation because it exceeded search pattern filesize limit: F053n01_R1_001_fastqc.html [2023-04-04 15:14:32,030] multiqc [DEBUG ] File ignored by rockhopper because it exceeded search pattern filesize limit: F053n01_R1_001_fastqc.html [2023-04-04 15:14:32,030] multiqc [DEBUG ] File ignored by rseqc/bam_stat because it exceeded search pattern filesize limit: F053n01_R1_001_fastqc.html [2023-04-04 15:14:32,030] multiqc [DEBUG ] File ignored by rseqc/junction_annotation because it exceeded search pattern filesize limit: F053n01_R1_001_fastqc.html [2023-04-04 15:14:32,030] multiqc [DEBUG ] File ignored by rseqc/read_distribution because it exceeded search pattern filesize limit: F053n01_R1_001_fastqc.html [2023-04-04 15:14:32,030] multiqc [DEBUG ] File ignored by rseqc/infer_experiment because it exceeded search pattern filesize limit: F053n01_R1_001_fastqc.html [2023-04-04 15:14:32,070] multiqc [DEBUG ] File ignored by nanostat because it exceeded search pattern filesize limit: F053n01_R1_001_fastqc.html [2023-04-04 15:14:33,267] multiqc.plots.linegraph [DEBUG ] Using matplotlib version 3.4.3 [2023-04-04 15:14:33,276] multiqc.plots.bargraph [DEBUG ] Using matplotlib version 3.4.3 [2023-04-04 15:14:38,952] multiqc.modules.fastqc.fastqc [INFO ] Found 64 reports [2023-04-04 15:14:39,952] multiqc [INFO ] Compressing plot data [2023-04-04 15:14:40,629] multiqc [INFO ] Report : multiqc_report.html [2023-04-04 15:14:40,629] multiqc [INFO ] Data : multiqc_data [2023-04-04 15:14:40,629] multiqc [DEBUG ] Moving data file from '/tmp/tmpecqnbhwg/multiqc_data' to '/home/shared/8TB_HDD_02/mattgeorgephd/USDA-NRSP-8-gigas-rDNA/fastqc/untrimmed/multiqc_data' [2023-04-04 15:14:40,838] multiqc [INFO ] MultiQC complete