FastQCFastQC Report
Tue 9 Aug 2022
T137F_S135_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT137F_S135_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4922379
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA107890.21918263506324887No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG106620.21660258180038555No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG86280.17528109883452697No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG83080.16878017722731226No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT70840.14391415207971592No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG68940.1400542298754322No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA66380.13485349258966042No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT64140.1303028474646101No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC60420.12274552609622298No Hit
TTGGTGGAAAATGTGTTAAGAGACGACCATGGGCATTGATTAAAACCAAA56700.11518820472783586No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA55460.11266909760504015No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT54590.11090165954307867No Hit
GCATTGGTTAAAACCAAATGTAAATGTACTTGTTCATCATCCGGATAAAA51630.10488830705640505No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG50470.10253172297378971No Hit
AATTCTCGATGGTAAAGAAATTGATGCGGCTAAGGAATGTTGAAGAATTA49840.10125185403236932No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC49690.10094712333203112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)