FastQCFastQC Report
Tue 9 Aug 2022
T135G_S145_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT135G_S145_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4370992
Sequences flagged as poor quality0
Sequence length20-86
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG117250.2682457437579387No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG102310.23406585964925122No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG94610.21644972125320752No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC88120.20160183317654207No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG86270.197369384341129No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG79090.18094290723936352No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA66620.15241391427849788No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT65060.14884493039566304No Hit
AAGGGCTGATGGCTACATCCTAGAAGGAAAAGAACTTGAATTCTACATGA64600.14779253771226303No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA61880.14156969401911512No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG58700.13429445764256717No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA53630.12269526002335396No Hit
ACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCT52290.1196295943804061No Hit
ATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGA46240.1057883427835146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)