FastQCFastQC Report
Tue 9 Aug 2022
T135F_S133_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT135F_S133_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5487294
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA138290.252018572360074No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG126930.23131620066284037No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG98230.1790135538573293No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG96930.17664444442014587No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG93100.16966468353982855No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA90530.1649811364217044No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT87150.1588214518850275No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC85670.1561243119103879No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT83610.15237018464838953No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG71880.130993527957496No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC67940.12381330397095544No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT67940.12381330397095544No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA67490.12299322762731504No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA67150.12237361438989783No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG60200.10970799086034028No Hit
ACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCT56070.102181512417596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)