FastQCFastQC Report
Tue 9 Aug 2022
T134G_S144_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT134G_S144_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5184462
Sequences flagged as poor quality0
Sequence length20-86
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG124960.24102790222013393No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG100790.19440782862329783No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG96880.18686606247668514No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG93440.1802308513400233No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC87490.16875425068213443No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG77890.15023738239377588No Hit
AAGGGCTGATGGCTACATCCTAGAAGGAAAAGAACTTGAATTCTACATGA67260.12973380844531215No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT66420.12811358247008078No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA66120.12753493033606958No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA65870.1270527202243936No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA64170.12377369146499674No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG58960.11372443273766882No Hit
ACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCT54630.10537255360344043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)