FastQCFastQC Report
Tue 9 Aug 2022
T134F_S132_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT134F_S132_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5473605
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA113810.20792512430107762No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG102260.18682385740293644No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG81440.1487867685008326No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG79050.14442035916000515No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT74900.13683851867279426No Hit
ATGCCATTGCAGTACTTGATGTAATAAAATCGTTCAAGCAG73050.13345866207006168No Hit
ACGTAGGGGTAGAATATACAGAGGCTAGAAAGTGAAGATATTCTAAGTCT71270.13020669193337847No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA70070.12801435251538976No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT68880.12584028259255098No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG60500.11053044565692993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)