FastQCFastQC Report
Tue 9 Aug 2022
T133G_S143_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT133G_S143_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5900041
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG175300.2971165793593638No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG142100.24084578395302675No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG132860.2251848758339137No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG126670.21469342331688884No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC124830.21157480092087494No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG109260.18518515379808376No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA100690.170659831007954No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT97930.16598189741393324No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA96170.16299886729600693No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG86560.14671084489073888No Hit
ACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCT80240.13599905492182174No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA80110.13577871746992945No Hit
AAGGGCTGATGGCTACATCCTAGAAGGAAAAGAACTTGAATTCTACATGA75120.12732114912421796No Hit
ATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGA67620.11460937305350928No Hit
GAATAAGAGTGATGATGTATCCATCAAGAGTATAAATAAAAAGAAAAAAGACT62450.10584672208210077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)