FastQCFastQC Report
Tue 9 Aug 2022
T133F_S131_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT133F_S131_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819960
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA86050.22526413889150673No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT53260.13942554372297092No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT51800.13560351417292327No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG50640.13256683316055665No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA49540.12968722185572626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)