FastQCFastQC Report
Tue 9 Aug 2022
T030F_S196_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT030F_S196_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4636056
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG130870.28228735804744376No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG109100.23532934028406902No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG99720.21509662523489792No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA80380.17338013173266242No Hit
ACGTAGGGGTAGAATATACAGAGGCTAGAAAGTGAAGATATTCTAAGTCT66550.14354874056741335No Hit
TTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAAGG62340.13446774585984292No Hit
AAGGGCTGATGGCTACATCCTAGAAGGAAAAGAACTTGAATTCTACATGA60440.13036943470915796No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG52600.11345850869791047No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT51870.11188389441369993No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT48840.10534816663129178No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT48290.10416181340346191No Hit
CAGACGTAGGGGTAGAATATACAGAGGCTAGAAAGTGAAGATATTCTAAG48030.10360099187757871No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC47950.10342843140807617No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA46540.10038705313309418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)