FastQCFastQC Report
Tue 9 Aug 2022
T029F_S195_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT029F_S195_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4792035
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA131380.2741632730144917No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT85300.1780037082366886No Hit
TACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACTA84910.17718985775354312No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT81990.17109641311050525No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA78530.1638760985677275No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC60180.12558338993767784No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG59570.12431044431019389No Hit
GAATAAGAGTGATGATGTATCCATCAAGAGTATAAAT53530.11170619580199226No Hit
GACTGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATC49310.10289991621513617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)