FastQCFastQC Report
Tue 9 Aug 2022
T019F_S175_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT019F_S175_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4853716
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG109360.22531190535251752No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA109360.22531190535251752No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG87050.17934712290541927No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG82840.17067335624910893No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG70660.14557918098215883No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA68240.14059331036261702No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT67240.13853303324710387No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT65270.13447428732954297No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC63320.13045674695429235No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG54410.11209967785507022No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC52920.10902986495295564No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA49380.1017364839640391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)