FastQCFastQC Report
Tue 9 Aug 2022
T016F_S173_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT016F_S173_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4047786
Sequences flagged as poor quality0
Sequence length20-86
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA102610.25349660283424075No Hit
TGATCTGGAGGGGTATAAATAAGGAAAGTAGGTTAAATAGATTTAATGAA93780.23168220849620014No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG84950.20986781415815955No Hit
ACGTAGGGGTAGAATATACAGAGGCTAGAAAGTGAAGATATTCTAAGTCT69950.17281051913317552No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG67500.16675782761242813No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT66090.16327444188007964No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA63360.15653001418553253No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT61970.15309603817988401No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG59350.14662336398218678No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC48580.12001622615424828No Hit
CAGACGTAGGGGTAGAATATACAGAGGCTAGAAAGTGAAGATATTCTAAG46890.11584110424810007No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG44450.10981311759070268No Hit
ATTCAGACGTAGGGGTAGAATATACAGAGGCTAGAAAGTGAAGATATTCT41410.10230283913230591No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA41340.10212990508885599No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT40930.10111700569150643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)