FastQCFastQC Report
Tue 9 Aug 2022
T015F_S172_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT015F_S172_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5230498
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA133990.2561706361421035No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG132070.2524998575661438No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG102960.1968455011358383No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG96740.18495370804080224No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA92410.1766753376064765No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT82940.15856998702609196No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT79090.15120931123575612No Hit
ACGTAGGGGTAGAATATACAGAGGCTAGAAAGTGAAGATATTCTAAGTCT77780.1487047696032003No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG75570.14447955051316339No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC71160.13604823097150598No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC66990.12807575875184352No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG57660.1102380691092894No Hit
GACTGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATC55410.10593637546558665No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT54130.10348918974828018No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA52860.10106112266939017No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA52420.10021990257906609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)