FastQCFastQC Report
Tue 9 Aug 2022
T014F_S171_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT014F_S171_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4310005
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA121630.28220384895145134No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG106510.24712268315233973No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG92620.2148953423487908No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG85100.19744756676616385No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC82840.19220395335968288No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG82480.19136868750732308No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT80250.18619467958853875No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA75090.17422253570471496No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT74470.1727840222923175No Hit
TACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACTA72680.16863089485975075No Hit
ACGTAGGGGTAGAATATACAGAGGCTAGAAAGTGAAGATATTCTAAGTCT70560.1637121070625208No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG69600.16148473145622802No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA63550.14744762477073695No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC59160.1372620217377938No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA55680.12918778516498242No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT54560.12658918029097413No Hit
ACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCT53160.1233409241984638No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG49550.11496506384563358No Hit
CAGACGTAGGGGTAGAATATACAGAGGCTAGAAAGTGAAGATATTCTAAG48300.1120648351916065No Hit
ATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGA45740.10612516690815903No Hit
GACTGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATC45340.10519709373887037No Hit
TTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAAGG43820.10167041569557345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)