FastQCFastQC Report
Tue 9 Aug 2022
T012FX_S230_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT012FX_S230_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4720330
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG203370.430838521883004No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG158040.3348071003510348No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG149600.31692699451097694No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA98650.20898962572532004No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG62940.13333813525749258No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT60870.12895284863558268No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT58950.1248853364065648No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT58850.12467348681130346No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG56770.1202670152298674No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA56750.12022464531081514No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC55020.11655964731279382No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA53440.1132124237076645No Hit
CTGCCATTGGATTAAACTTACACTATGTAATGAATACTAAGAAAAAATGAAAATGACTTACTACT51020.10808566350233988No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG47410.1004378931134052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)