FastQCFastQC Report
Tue 9 Aug 2022
T010F_S109_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT010F_S109_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5203960
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA175970.3381463347143329No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT114320.21967885994511874No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA111010.21331831912620391No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT106350.20436360002767123No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC86080.16541249356259463No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG68110.13088109824057065No Hit
GACTGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATC65940.12671119685777754No Hit
TACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACTA65770.12638452255589974No Hit
TTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAAGG65630.12611549666023567No Hit
GTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAGTCA60340.11595016103121468No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG58070.11158809829437581No Hit
GCCGTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAG57010.10955118794149071No Hit
AAGGGCTGATGGCTACATCCTAGAAGGAAAAGAACTTGAATTCTACATGA56990.10951275567068156No Hit
CGGGCGTTATGGCGGAGGCTGGAATGGACGACGAAAATGGTATTGAAACG56920.10937824272284952No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG54800.1053044220170793No Hit
AAGTCCTCCAAGCTTGGCACAGGGCGTCCAGTGCTATTGGGTTGTAGGGG53400.10261416306043859No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC52040.10000076864541618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)