FastQCFastQC Report
Tue 9 Aug 2022
T010FX_S228_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT010FX_S228_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3970562
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA90950.2290607727571059No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG78590.19793167818560695No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG65410.1647373847833128No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG62020.15619955059258614No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT60750.15300101094001303No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA60290.15184248476664008No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT57640.14516836659394816No Hit
AAGGGCTGATGGCTACATCCTAGAAGGAAAAGAACTTGAATTCTACATGA53460.13464088962721146No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC45300.11408964272563935No Hit
GTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAGTCA44210.11134443940177738No Hit
GCCGTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAG44140.11116814194061195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)