FastQCFastQC Report
Tue 9 Aug 2022
T009FX_S227_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT009FX_S227_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4421028
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG207510.4693704722069166No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG168800.38181165104586534No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG163290.36934848637013834No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA139270.31501723128647907No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA86780.19628918884929025No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT86220.19502251512544141No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT85320.19298678949782722No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG67390.15243061116102408No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC65800.14883416255223897No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC58850.13311383687232925No Hit
GCCGTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAG57160.12929119652714255No Hit
GTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAGTCA55370.12524236444555428No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT54660.12363640311710308No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG53700.12146496244764791No Hit
GACTGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATC52750.11931614095183293No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG52170.11800422888070376No Hit
ATCGCCGTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCAT51070.11551611978028639No Hit
TTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAAGG49470.11189705199786114No Hit
TACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACTATTATATAA48980.11078871248949339No Hit
TGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACT48660.11006489893300835No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCCA48000.10857203347275793No Hit
TACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACTA47910.10836846090999651No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA47810.10814226917359492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)