FastQCFastQC Report
Tue 9 Aug 2022
T007F_S106_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT007F_S106_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5271072
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA191290.36290530654864894No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA128310.24342296974884806No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT122220.2318693427067587No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT116070.22020188682681627No Hit
AAAGAAATACTATTATAAATATAAAAACAGCGGAAAATACAAGTACCTGA113240.2148329599747452No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG105510.2001680113646712No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC97160.18432683143011516No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG92070.17467035168557743No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG90510.17171080190139693No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG79590.15099395341213326No Hit
GACTGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATC76490.14511279678972325No Hit
TACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACTA75640.14350022158680437No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC70100.1329900255583684No Hit
AAAAGCAAAGAAATACTATTATAAATATAAAAACAGCGGAAAATACAAGT67270.12762109870629731No Hit
AGCAAAGAAATACTATTATAAATATAAAAACAGCGGAAAATACAAGTACC66810.12674841094942357No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG60670.11509992654245664No Hit
AAGCGTGATTGGGACGGGCACGGGCTGGGCGAGGCTGGCTCTTCTTTCGG58710.11138151783925546No Hit
CGGGCGTTATGGCGGAGGCTGGAATGGACGACGAAAATGGTATTGAAACG55460.10521578912221272No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA54900.10415338663558381No Hit
GTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAGTCA54380.10316687004085696No Hit
TTTTAGTCCTTATTGGATCATTTGCTGTACAAAGTGATGCAGCTGCTTAT54260.10293921236515077No Hit
AGCATACAGAGTGACATGTGGACTACAGAAATTTTATAACTACTGGGGTA53280.10108000801355019No Hit
TGTCCGCACAAAAGAGCTTGTCACAAATGTTGTGACCACCTT53170.1008713218108195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)