FastQCFastQC Report
Tue 9 Aug 2022
T007FX_S225_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT007FX_S225_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4190079
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG229240.547101856552108No Hit
TTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAAGG206070.49180456979450743No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG194150.46335641881692446No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG192010.45824911654410333No Hit
ACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTA140660.33569772789486785No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGG132250.31562650728065034No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA122390.2920947313881194No Hit
TTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAAGGT108480.2588972666147822No Hit
CTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAA102850.24546076577553788No Hit
CCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGT95490.22789546450078865No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT84120.20075993793911762No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA80760.19274099605281905No Hit
GCCGTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAG79130.18885085460202541No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT78310.18689385092739302No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG75960.18128536478667825No Hit
ATCGCCGTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCAT74150.1769656371634043No Hit
GTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAGTCA74140.17694177126493318No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC69720.1663930441406952No Hit
TACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACTATTATATAA66420.15851729764522338No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT65710.15682281885377342No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG65400.15608297600116847No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC62880.15006876958644455No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG60050.1433147203191157No Hit
TGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACT58650.13997349453315797No Hit
TTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACTATTATA58290.13911432218819741No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA57900.13818355214782346No Hit
CCAAACGTTCGGTTAAGGTGCCAAACACGGACGCTCATCAGATACCATAA56200.13412634940773194No Hit
ACCAAACGTTCGGTTAAGGTGCCAAACACGGACGCTCATCAGATACCATA56060.13379222682913616No Hit
AAACGTTCGGTTAAGGTGCCAAACACGGACGCTCATCAGATACCATAAAA53470.12761095912511433No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA52810.12603580982602No Hit
TACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACTA51940.12395947665903198No Hit
GACTGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATC48470.11567800988955101No Hit
ACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCT47830.1141505923873989No Hit
AACCAAACGTTCGGTTAAGGTGCCAAACACGGACGCTCATCAGATACCAT46040.10987859656106723No Hit
CTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACTATT45020.10744427491701229No Hit
GAACCAAACGTTCGGTTAAGGTGCCAAACACGGACGCTCATCAGATACCA44820.10696695694758977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)