FastQCFastQC Report
Tue 9 Aug 2022
T006F_S105_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT006F_S105_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4159998
Sequences flagged as poor quality0
Sequence length20-86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA170800.41057712046976946No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG139680.33576939219682317No Hit
TACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACTA115410.27742801799423944No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT112410.2702164760656135No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT101750.24459146374589602No Hit
TTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAAGG98350.23641838289345332No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG97340.2339904971108159No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG92930.2233895304757358No Hit
TGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACT81070.19487990138456798No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA73290.1761779693163314No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGG72780.174952007188465No Hit
ACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTA70560.16961546616128179No Hit
TTTTGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTC65770.15810103754857574No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC59410.14281256865988878No Hit
CGCGTTTATTAGATCAAAACCAGTCGGGTCCGCAAGGGCCCGTCGGATTG56710.13632218092412546No Hit
AGTTTTTGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCT55860.13427891071101478No Hit
AGTCCGACTCGCTTAATTGGAGTCGCGACCTTCGAATGTATGGTGCCAAG55200.13269237148671706No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT54790.13170679408980485No Hit
TACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACTATTATATAA54590.13122602462789645No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA50700.12187505859377817No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG50230.12074525035829344No Hit
GACTGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATC50100.12043275020805297No Hit
TTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACTATTATA48680.11701928702850337No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC47570.11435101651491178No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG46750.11237986172108737No Hit
CCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGT46490.11175486142060646No Hit
ATCAGTGAAAATAAGTCTTTCACTATTATATAAATCTAACTCGTATTTCC46230.11112986112012553No Hit
CTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAA46060.11072120707750341No Hit
TTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAAGGT45420.10918274479939653No Hit
GTGAAAATAAGTCTTTCACTATTATATAAATCTAACTCGTATTTCCTAAC44600.10721159000557212No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG43440.10442312712650341No Hit
AAAATAAGTCTTTCACTATTATATAAATCTAACTCGTATTTCCTAACTTT43030.10343754972959121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)