FastQCFastQC Report
Tue 9 Aug 2022
T006FX_S224_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT006FX_S224_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4848955
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG256230.5284231344691794No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG208590.4301751614523129No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG195550.40328276917397665No Hit
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA148660.30658152117311877No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT94760.19542355002263373No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT90470.18657628293106454No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA88650.18282289689221698No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG75030.15473437060150075No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC66740.1376379034245523No Hit
AGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTT66160.13644176941217231No Hit
GGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAG64620.13326582737930132No Hit
AACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACC63570.13110041235688927No Hit
TTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAGAAGCTTCTA62090.12804820832529895No Hit
GTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAGTCA59170.12202629226297212No Hit
GCCGTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCATTAG57650.11889159623052802No Hit
GATAACAGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCG57590.11876785822924733No Hit
GACTGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATC52430.10812639011910814No Hit
ATCGCCGTAAGACGAACTACTGCGAAAGCATTTGCCAAGCATGTTTTCAT50460.10406365907705886No Hit
TTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAAGG49770.10264067206233096No Hit
GCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCGA49620.10233132705912923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)