FastQCFastQC Report
Tue 9 Aug 2022
T005F_S104_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT005F_S104_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3928545
Sequences flagged as poor quality0
Sequence length20-86
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA154880.3942426521778419No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT98290.2501944103987609No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT94540.24064889163799827No Hit
TACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACTA84480.21504144664245922No Hit
TTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTAAGG74860.19055400918151633No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG67890.17281207164484563No Hit
TTGGTGGAAAATGTGTTAAGAGACGACCATGGGCATTGATTAAAACCAAA66010.16802658490611663No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA64740.16479383588580504No Hit
GTATTGGTGGAAAATGTGTTAAGAGACGACCATGGGCATTGATTAAAACC63400.16138290384862589No Hit
TGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCC56690.14430278894603474No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGG55900.14229186632710072No Hit
TGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTCACT55430.1410954946424185No Hit
ACTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGTTA54380.138422749389405No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG51470.13101542683105322No Hit
TTTTGGCTCTTATACGATTTTGATGGGAGATCAGTGAAAATAAGTCTTTC47830.12174990995393968No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG44760.11393531192846207No Hit
GAGGTAGTGACGAAAAATAACAATACGGGACTCTTTCGAGGCCCCGTAAT44390.11299348741073349No Hit
GACTGACGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATC43780.1114407496923161No Hit
GATGTATTGGTGGAAAATGTGTTAAGAGACGACCATGGGCATTGATTAAA41070.10454252146787169No Hit
AGTGACGAAAAATAACAATACGGGACTCTTTCGAGGCCCCGTAATTGGAA40810.10388069883379214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)