FastQCFastQC Report
Tue 9 Aug 2022
T004FX_S222_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameT004FX_S222_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5514914
Sequences flagged as poor quality0
Sequence length20-86
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTTAGA103830.18827129489235916No Hit
AGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCGTAG95550.17325746149441315No Hit
AAGGGCTGATGGCTACATCCTAGAAGGAAAAGAACTTGAATTCTACATGA77590.14069122383413415No Hit
TGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAGGCG77310.14018350966125673No Hit
TATTGGAGGGGAAGATTGCGACCTCGATGTTGGCTTTAGATATCCTAGAG71520.12968470587211334No Hit
CGGGGGCATTCGTATTACGGTGTTAGAGGTGAAATTCTTGGATCGCCGTA68470.12415424791755593No Hit
AGCTCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGT66870.12125302407254221No Hit
TCGAGGTAATGATTAAGAGGGACTGACGGGGGCATTCGTATTACGGTGTT64630.117191310689523No Hit
AGCGCAATTTCTCCCGAAAGATGGTATTGGAGGGGAAGATTGCGACCTCG58100.10535069087206075No Hit
AGCAGCCAGAGCAGCAGCATCAGCAGCAGCTAGAGCAACCGTAGCAGGAC55550.10072686536907012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)